Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin

A simple method for concentrating, isolating, and purifying PF/cholera enterotoxin from culture supernatant fluids is presented. Precipitation with dextran sulfate and ammonium sulfate, followed by gel filtration and ion-exchange chromatography, yielded PF/enterotoxin of high biological potency. Alt...

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Detalhes bibliográficos
Publicado no:Infect Immun
Main Authors: Richardson, Stephen H., Evans, Dolores G., Feeley, John C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1970
Assuntos:
Pathogenic Microorganisms and Infection
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC415940/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16557774/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.1.6.546-554.1970
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