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Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin

A simple method for concentrating, isolating, and purifying PF/cholera enterotoxin from culture supernatant fluids is presented. Precipitation with dextran sulfate and ammonium sulfate, followed by gel filtration and ion-exchange chromatography, yielded PF/enterotoxin of high biological potency. Alt...

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Bibliografiske detaljer
Main Authors: Richardson, Stephen H., Evans, Dolores G., Feeley, John C.
Format: Artigo
Sprog:Inglês
Udgivet: 1970
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC415940/
https://ncbi.nlm.nih.gov/pubmed/16557774
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