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Pulse-chase analysis for studies of MHC class II biosynthesis, maturation, and peptide loading

Pulse-chase analysis is a commonly used technique for studying the synthesis, processing and transport of proteins. Cultured cells expressing proteins of interest are allowed to take up radioactively labeled amino acids for a brief interval (“pulse”), during which all newly synthesized proteins inco...

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Bibliografiske detaljer
Main Authors: Hou, Tieying, Rinderknecht, Cornelia H, Hadjinicolaou, Andreas V, Busch, Robert, Mellins, Elizabeth
Format: Artigo
Sprog:Inglês
Udgivet: 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4159250/
https://ncbi.nlm.nih.gov/pubmed/23329504
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-218-6_31
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