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Pulse-chase analysis for studies of MHC class II biosynthesis, maturation, and peptide loading
Pulse-chase analysis is a commonly used technique for studying the synthesis, processing and transport of proteins. Cultured cells expressing proteins of interest are allowed to take up radioactively labeled amino acids for a brief interval (“pulse”), during which all newly synthesized proteins inco...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4159250/ https://ncbi.nlm.nih.gov/pubmed/23329504 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-218-6_31 |
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