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Guanidine extraction of streptococcal M protein.
A new method of extracting M protein from streptococcal cell walls has been presented. The extracting agent was guanidine-hydrochloride, a protein denaturant. The crude guanidine extract was further purified by ammonium sulfate and pH 5 fractionation and by hydroxyapatite column chromatography. Thre...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1975
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC415339/ https://ncbi.nlm.nih.gov/pubmed/809363 |
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