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Guanidine extraction of streptococcal M protein.

A new method of extracting M protein from streptococcal cell walls has been presented. The extracting agent was guanidine-hydrochloride, a protein denaturant. The crude guanidine extract was further purified by ammonium sulfate and pH 5 fractionation and by hydroxyapatite column chromatography. Thre...

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Detalhes bibliográficos
Main Authors: Russell, H, Facklam, R R
Formato: Artigo
Idioma:Inglês
Publicado em: 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC415339/
https://ncbi.nlm.nih.gov/pubmed/809363
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