Competitive binding-based optical DNA mapping for fast identification of bacteria - multi-ligand transfer matrix theory and experimental applications on Escherichia coli

We demonstrate a single DNA molecule optical mapping assay able to resolve a specific Escherichia coli strain from other strains. The assay is based on competitive binding of the fluorescent dye YOYO-1 and the AT-specific antibiotic netropsin. The optical map is visualized by stretching the DNA mole...

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Detalhes bibliográficos
Main Authors: Nilsson, Adam N., Emilsson, Gustav, Nyberg, Lena K., Noble, Charleston, Stadler, Liselott Svensson, Fritzsche, Joachim, Moore, Edward R. B., Tegenfeldt, Jonas O., Ambjörnsson, Tobias, Westerlund, Fredrik
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4150756/
https://ncbi.nlm.nih.gov/pubmed/25013180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku556
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