Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method

AIM: To enhance the differentiation of insulin producing cell (IPC) ability from embryonic stem (ES) cells in vitro. METHODS: Four-day embryoid body (EB)-formatted ES cells were dissociated as single cells for the followed plasmid DNA delivery. The use of Nucleofector™electroporator (Amaxa biosystem...

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Detalhes bibliográficos
Main Authors: Lin, Han-Tso, Kao, Chung-Lan, Lee, Kun-Hsiung, Chang, Yuh-Lih, Chiou, Shih-Hwa, Tsai, Fu-Ting, Tsai, Tung-Hu, Sheu, Dey-Chyi, Ho, Larry LT, Ku, Hung-Hai
Formato: Artigo
Idioma:Inglês
Publicado em: Baishideng Publishing Group Co., Limited 2007
Assuntos:
Basic Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4146945/
https://ncbi.nlm.nih.gov/pubmed/17461469
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v13.i11.1672
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