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Profiling thiol metabolites and quantification of cellular glutathione using FT-ICR-MS spectrometry

We describe preparation and use of the quaternary ammonium-based α-iodoacetamide QDE and its isotopologue *QDE as reagents for chemoselective derivatization of cellular thiols. Direct addition of the reagents to live cells followed by adduct extraction into n-butanol and analysis by FT-ICR-MS provid...

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Main Authors: Gori, Sadakatali S., Lorkiewicz, Pawel, Ehringer, Daniel S., Belshoff, Alex C., Higashi, Richard M., Fan, Teresa W.-M., Nantz, Michael H.
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2014
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4144182/
https://ncbi.nlm.nih.gov/pubmed/24858467
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-014-7810-z
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