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Profiling thiol metabolites and quantification of cellular glutathione using FT-ICR-MS spectrometry
We describe preparation and use of the quaternary ammonium-based α-iodoacetamide QDE and its isotopologue *QDE as reagents for chemoselective derivatization of cellular thiols. Direct addition of the reagents to live cells followed by adduct extraction into n-butanol and analysis by FT-ICR-MS provid...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4144182/ https://ncbi.nlm.nih.gov/pubmed/24858467 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-014-7810-z |
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