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Down Regulation of ackA-pta Pathway in Escherichia coli BL21 (DE3): A Step Toward Optimized Recombinant Protein Expression System

BACKGROUND: One of the most important problems in production of recombinant protein is to attain over-expression of the target gene and high cell density. In such conditions, the secondary metabolites of bacteria become toxic for the medium and cause cells to die. One of these aforementioned metabol...

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Detalhes bibliográficos
Main Authors: Bakhtiari, Nahid, Mirshahi, Manouchehr, Babaeipour, Valiollah, Maghsoudi, Nader, Tahzibi, Abbas
Formato: Artigo
Idioma:Inglês
Publicado em: Kowsar 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4138692/
https://ncbi.nlm.nih.gov/pubmed/25147677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5812/jjm.8990
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