Detection of Hepatitis B Virus Genotypic Resistance Mutations by Coamplification at Lower Denaturation Temperature-PCR Coupled with Sanger Sequencing

Mutations in the reverse transcriptase (rt) region of the DNA polymerase gene are the primary cause of hepatitis B virus (HBV) drug resistance. In this study, we established a novel method that couples coamplification at lower denaturation temperature (COLD)-PCR and Sanger sequencing, and we applied...

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Detalhes bibliográficos
Main Authors: Liu, Can, Lin, Jinpiao, Chen, Huijuan, Shang, Hongyan, Jiang, Ling, Chen, Jing, Ye, Yang, Yang, Bin, Ou, Qishui
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4136161/
https://ncbi.nlm.nih.gov/pubmed/24899029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01127-14
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