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Coamplification at Lower Denaturation Temperature–PCR Increases Mutation-Detection Selectivity of TaqMan-Based Real-Time PCR
BACKGROUND: DNA genotyping with mutation-specific TaqMan® probes (Applied Biosystems) is broadly used in detection of single-nucleotide polymorphisms but is less so for somatic mutations because of its limited selectivity for low-level mutations. We recently described coamplification at lower denatu...
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| Glavni autori: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2009
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2754313/ https://ncbi.nlm.nih.gov/pubmed/19233916 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1373/clinchem.2008.113381 |
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