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Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres

Three-dimensional cell culture has many advantages over monolayer cultures, and spheroids have been hailed as the best current representation of small avascular tumours in vitro. However their adoption in regular screening programs has been hindered by uneven culture growth, poor reproducibility and...

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Autors principals: Ivanov, Delyan P., Parker, Terry L., Walker, David A., Alexander, Cameron, Ashford, Marianne B., Gellert, Paul R., Garnett, Martin C.
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4131917/
https://ncbi.nlm.nih.gov/pubmed/25119185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0103817
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