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Protein crystallization with microseed matrix screening: application to human germline antibody Fabs

The crystallization of 16 human antibody Fab fragments constructed from all pairs of four different heavy chains and four different light chains was enabled by employing microseed matrix screening (MMS). In initial screening, diffraction-quality crystals were obtained for only three Fabs, while many...

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Bibliografische gegevens
Hoofdauteurs: Obmolova, Galina, Malia, Thomas J., Teplyakov, Alexey, Sweet, Raymond W., Gilliland, Gary L.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: International Union of Crystallography 2014
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4118815/
https://ncbi.nlm.nih.gov/pubmed/25084393
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14012552
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