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Characterization of Genomic Deletion Efficiency Mediated by Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9 Nuclease System in Mammalian Cells
The clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 nuclease system has provided a powerful tool for genome engineering. Double strand breaks may trigger nonhomologous end joining repair, leading to frameshift mutations, or homology-directed repair using an ext...
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Main Authors: | , , , , , , , , |
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格式: | Artigo |
語言: | Inglês |
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American Society for Biochemistry and Molecular Biology
2014
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4118095/ https://ncbi.nlm.nih.gov/pubmed/24907273 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M114.564625 |
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