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Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase
Synchronous cultures are often indispensable for studying meiosis. Here, we present an optimized protocol for induction of synchronous meiosis in the fission yeast Schizosaccharomyces pombe. Chemical inactivation of an ATP analog-sensitive form of the Pat1 kinase (pat1-as2) by adding the ATP-analog...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4117242/ https://ncbi.nlm.nih.gov/pubmed/24385151 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.013 |
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