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Measuring Fate and Rate of Single-Molecule Competition of Amplification and Restriction Digestion, and Its Use for Rapid Genotyping Tested with Hepatitis C Viral RNA()

We experimentally monitored, at the single-molecule level, the competition among reverse transcription, exponential amplification (RT-LAMP), and linear degradation (restriction enzymes) starting with Hepatitis C viral RNA molecules. We found significant heterogeneity in the rate of single-molecule a...

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Detalhes bibliográficos
Main Authors: Sun, Bing, Rodriguez-Manzano, Jesus, Selck, David A., Khorosheva, Eugenia, Karymov, Mikhail A., Ismagilov, Rustem F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4116457/
https://ncbi.nlm.nih.gov/pubmed/24889060
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201403035
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