Critical comparison of sample preparation strategies for shotgun proteomic analysis of formalin-fixed, paraffin-embedded samples: insights from liver tissue

BACKGROUND: The growing field of formalin-fixed paraffin-embedded (FFPE) tissue proteomics holds promise for improving translational research. Direct tissue trypsinization (DT) and protein extraction followed by in solution digestion (ISD) or filter-aided sample preparation (FASP) are the most commo...

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Bibliographic Details
Main Authors: Tanca, Alessandro, Abbondio, Marcello, Pisanu, Salvatore, Pagnozzi, Daniela, Uzzau, Sergio, Addis, Maria Filippa
Format: Artigo
Language:Inglês
Published: Springer 2014
Subjects:
Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4115481/
https://ncbi.nlm.nih.gov/pubmed/25097466
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1559-0275-11-28
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