Optimization of Urea Based Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue for Shotgun Proteomics

Urea based protein extraction of formalin-fixed paraffin-embedded (FFPE) tissue provides the most efficient workflow for proteomics due to its compatibility with liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). This study optimizes the use of urea for proteomic...

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Bibliographic Details
Published in:Int J Proteomics
Main Authors: Luebker, Stephen A., Koepsell, Scott A.
Format: Artigo
Language:Inglês
Published: Hindawi Publishing Corporation 2016
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5021876/
https://ncbi.nlm.nih.gov/pubmed/27660725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2016/4324987
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