Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase.
RNA ligase induced by bacteriophage T4 catalyzed the addition of nucleoside 5',3'-diphosphates to oligoribonucleotide acceptors in the presence of ATP. The reactions proceeded in equimolar concentrations of donors and acceptors. Several oligonucleotides of defined sequence were synthesized by this m...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1978
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC411452/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/274717/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.3.1270 |
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