Replica plating and in situ enzymatic assay of animal cell colonies established on filter paper.
We have developed a simple technique for the replica plating of Chinese hamster ovary (CHO) cells. In this procedure cells are allowed to divide for 8-16 days between the plastic surface of a petri dish and a disc of Whatman no. 50 filter paper, weighted down with glass beads. The culture medium can...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1978
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC411435/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/349559/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.3.1190 |
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