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Replica plating and in situ enzymatic assay of animal cell colonies established on filter paper.

We have developed a simple technique for the replica plating of Chinese hamster ovary (CHO) cells. In this procedure cells are allowed to divide for 8-16 days between the plastic surface of a petri dish and a disc of Whatman no. 50 filter paper, weighted down with glass beads. The culture medium can...

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Detalhes bibliográficos
Main Authors: Esko, J D, Raetz, C R
Formato: Artigo
Idioma:Inglês
Publicado em: 1978
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC411435/
https://ncbi.nlm.nih.gov/pubmed/349559
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