Structural domains of IS10 transposase and reconstitution of transposition activity from proteolytic fragments lacking an interdomain linker.

All of the DNA cleavage and strand transfer events required for transposition of insertion sequence IS10 are carried out by a 46-kDa IS10-encoded transposase protein. Limited proteolysis demonstrates that transposase has two principal structural domains, a 28-kDa N-terminal domain (N alpha beta; aa...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Kwon, D, Chalmers, R M, Kleckner, N
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41131/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7667274/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.18.8234
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