Identification of residues in the Mu transposase essential for catalysis.
A tetramer of Mu transposase (MuA) cleaves the phage Mu DNA and joins these ends to a target DNA to catalyze transposition. Substitution mutations at Asp-269 or Glu-392 within MuA destroy both the DNA cleavage and joining activities without blocking tetramer assembly, indicating that the mutations s...
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| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1994
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC44261/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7912831/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.14.6654 |
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