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Time-Lapse Analysis of Human Embryonic Stem Cells Reveals Multiple Bottlenecks Restricting Colony Formation and Their Relief upon Culture Adaptation

Using time-lapse imaging, we have identified a series of bottlenecks that restrict growth of early-passage human embryonic stem cells (hESCs) and that are relieved by karyotypically abnormal variants that are selected by prolonged culture. Only a minority of karyotypically normal cells divided after...

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Bibliografische gegevens
Hoofdauteurs: Barbaric, Ivana, Biga, Veronica, Gokhale, Paul J., Jones, Mark, Stavish, Dylan, Glen, Adam, Coca, Daniel, Andrews, Peter W.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2014
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4110749/
https://ncbi.nlm.nih.gov/pubmed/25068128
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2014.05.006
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