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Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

Whole-mount immunofluorescence to detect activated Caspase 3 (Casp3 assay) is useful to identify cells undergoing either intrinsic or extrinsic apoptosis in zebrafish embryos. The whole-mount analysis provides spatial information in regard to tissue specificity of apoptosing cells, although sectioni...

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Detalhes bibliográficos
Main Authors: Sorrells, Shelly, Toruno, Cristhian, Stewart, Rodney A., Jette, Cicely
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4109746/
https://ncbi.nlm.nih.gov/pubmed/24378359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51060
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