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Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos

In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by i...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Bio Protoc
Main Authors: Narayanan, Rachna, Oates, Andrew C.
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7854236/
https://ncbi.nlm.nih.gov/pubmed/33654992
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3193
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