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Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos

In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by i...

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Bibliographic Details
Published in:Bio Protoc
Main Authors: Narayanan, Rachna, Oates, Andrew C.
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7854236/
https://ncbi.nlm.nih.gov/pubmed/33654992
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3193
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