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Multicolor multicycle molecular profiling (M3P) with quantum dots for single-cell analysis
Here we present a detailed protocol for molecular profiling of individual cultured mammalian cells using multicolor multicycle immunofluorescence with quantum dot probes. It includes instructions for cell culture growth and processing (2 h + 48–72 h for cell growth), preparation and characterization...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4108347/ https://ncbi.nlm.nih.gov/pubmed/24008381 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.112 |
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