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Multicolor multicycle molecular profiling (M3P) with quantum dots for single-cell analysis

Here we present a detailed protocol for molecular profiling of individual cultured mammalian cells using multicolor multicycle immunofluorescence with quantum dot probes. It includes instructions for cell culture growth and processing (2 h + 48–72 h for cell growth), preparation and characterization...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Zrazhevskiy, Pavel, True, Lawrence D., Gao, Xiaohu
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4108347/
https://ncbi.nlm.nih.gov/pubmed/24008381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.112
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