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The choice of reference gene affects statistical efficiency in quantitative PCR data analysis

Quantitative polymerase chain reaction (qPCR), a highly sensitive method of measuring gene expression, is widely used in biomedical research. To produce reliable results, it is essential to use stably expressed reference genes (RGs) for data normalization so that sample-to-sample variation can be co...

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Autors principals: Guo, Yi, Pennell, Michael L., Pearl, Dennis K., Knobloch, Thomas J., Fernandez, Soledad, Weghorst, Christopher M.
Format: Artigo
Idioma:Inglês
Publicat: 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4102495/
https://ncbi.nlm.nih.gov/pubmed/24107253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000114090
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