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Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis
BACKGROUND: Quantitative real-time polymerase chain reaction (RT-qPCR) is the key platform for the quantitative analysis of gene expression in a wide range of experimental systems and conditions. However, the accuracy and reproducibility of gene expression quantification via RT-qPCR is entirely depe...
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| Published in: | Plant Methods |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
BioMed Central
2018
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5861610/ https://ncbi.nlm.nih.gov/pubmed/29581727 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-018-0293-8 |
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