A method for noninvasive longitudinal measurements of [Ca(2+)] in arterioles of hypertensive optical biosensor mice

We used two-photon (2-p) Förster resonance energy transfer (FRET) microscopy to provide serial, noninvasive measurements of [Ca(2+)] in arterioles of living “biosensor” mice. These express a genetically encoded Ca(2+) indicator (GECI), either FRET-based exMLCK or intensity-based GCaMP2. The FRET rat...

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Detalhes bibliográficos
Main Authors: Mauban, Joseph R. H., Fairfax, Seth T., Rizzo, Mark A., Zhang, Jin, Wier, Withrow Gil
Formato: Artigo
Idioma:Inglês
Publicado em: American Physiological Society 2014
Assuntos:
Vascular Biology and Microcirculation
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4101644/
https://ncbi.nlm.nih.gov/pubmed/24858846
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajpheart.00182.2014
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