Single-molecule imaging analysis of Ras activation in living cells

A single-molecule fluorescence resonance energy transfer (FRET) method has been developed to observe the activation of the small G protein Ras at the level of individual molecules. KB cells expressing H- or K-Ras fused with YFP (donor) were microinjected with the fluorescent GTP analogue BodipyTR-GT...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Murakoshi, Hideji, Iino, Ryota, Kobayashi, Takeshi, Fujiwara, Takahiro, Ohshima, Chika, Yoshimura, Akihiko, Kusumi, Akihiro
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2004
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC409916/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15123831/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0401354101
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