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Single-molecule imaging analysis of Ras activation in living cells
A single-molecule fluorescence resonance energy transfer (FRET) method has been developed to observe the activation of the small G protein Ras at the level of individual molecules. KB cells expressing H- or K-Ras fused with YFP (donor) were microinjected with the fluorescent GTP analogue BodipyTR-GT...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC409916/ https://ncbi.nlm.nih.gov/pubmed/15123831 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401354101 |
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