Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates....

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Bibliographische Detailangaben
Hauptverfasser: Bossé, Janine T., Li, Yanwen, Angen, Øystein, Weinert, Lucy A., Chaudhuri, Roy R., Holden, Matthew T., Williamson, Susanna M., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., Langford, Paul R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2014
Schlagworte:
Clinical Veterinary Microbiology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4097740/
https://ncbi.nlm.nih.gov/pubmed/24759717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00685-14
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