Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates....

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Main Authors: Bossé, Janine T., Li, Yanwen, Angen, Øystein, Weinert, Lucy A., Chaudhuri, Roy R., Holden, Matthew T., Williamson, Susanna M., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., Langford, Paul R.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2014
主題:
Clinical Veterinary Microbiology
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4097740/
https://ncbi.nlm.nih.gov/pubmed/24759717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00685-14
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