Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates....

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Detalhes bibliográficos
Main Authors: Bossé, Janine T., Li, Yanwen, Angen, Øystein, Weinert, Lucy A., Chaudhuri, Roy R., Holden, Matthew T., Williamson, Susanna M., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., Langford, Paul R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Clinical Veterinary Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4097740/
https://ncbi.nlm.nih.gov/pubmed/24759717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00685-14
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