Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates....

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Autores principales: Bossé, Janine T., Li, Yanwen, Angen, Øystein, Weinert, Lucy A., Chaudhuri, Roy R., Holden, Matthew T., Williamson, Susanna M., Maskell, Duncan J., Tucker, Alexander W., Wren, Brendan W., Rycroft, Andrew N., Langford, Paul R.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2014
Materias:
Clinical Veterinary Microbiology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4097740/
https://ncbi.nlm.nih.gov/pubmed/24759717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00685-14
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