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Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
A unique open reading frame (ORF) Z3276 was identified as a specific genetic marker for E. coli O157:H7. A qPCR assay was developed for detection of E. coli O157:H7 by targeting ORF Z3276. With this assay, we can detect as low as a few copies of the genome of DNA of E. coli O157:H7. The sensitivity...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4089424/ https://ncbi.nlm.nih.gov/pubmed/24513664 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50967 |
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