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Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA

AIM: To rapidly quantify hepatitis B virus (HBV) DNA by real-time PCR using efficient TaqMan probe and extraction methods of virus DNA. METHODS: Three standards were prepared by cloning PCR products which targeted S, C and X region of HBV genome into pGEM-T vector respectively. A pair of primers and...

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Main Authors: Lu, Yan-Qin, Han, Jin-Xiang, Qi, Peng, Xu, Wei, Zu, Yan-Hui, Zhu, Bo
Formato: Artigo
Idioma:Inglês
Publicado: Baishideng Publishing Group Co., Limited 2006
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4087500/
https://ncbi.nlm.nih.gov/pubmed/17143958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v12.i45.7365
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