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An electroporation protocol for efficient DNA transfection in PC12 cells

A wide variety of mammalian cell types is used in gene transfection studies. Establishing transfection methods that enable highly efficient DNA uptake has become increasingly important. PC12 is an established rat pheochromocytoma cell line, which responds to exposure to NGF with cessation of growth,...

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Bibliografiske detaljer
Main Authors: Covello, Giuseppina, Siva, Kavitha, Adami, Valentina, Denti, Michela A.
Format: Artigo
Sprog:Inglês
Udgivet: Springer Netherlands 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4082779/
https://ncbi.nlm.nih.gov/pubmed/23846478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-013-9608-9
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