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Effects of Circular DNA Length on Transfection Efficiency by Electroporation into HeLa Cells

The ability to produce extremely small and circular supercoiled vectors has opened new territory for improving non-viral gene therapy vectors. In this work, we compared transfection of supercoiled DNA vectors ranging from 383 to 4,548 bp, each encoding shRNA against GFP under control of the H1 promo...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Hornstein, Benjamin D., Roman, Dany, Arévalo-Soliz, Lirio M., Engevik, Melinda A., Zechiedrich, Lynn
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5137892/
https://ncbi.nlm.nih.gov/pubmed/27918590
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0167537
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