Human papilloma virus detection by in situ hybridisation signal amplification based on biotinylated tyramine deposition

Aim—To describe a method for amplifying human papilloma virus (HPV) in situ hybridisation (ISH) signals. Methods—Three human cervical cell lines, namely CaSKi, HeLa and SiHa, containing different copy numbers of integrated HPV DNA were studied. Following ISH, catalysed reporter deposition (CARD), ba...

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Publicado en:Clin Mol Pathol
Autores principales: Poddighe, P J, Bulten, J, Kerstens, H M J, Robben, J C M, Melchers, W J G, Hanselaar, A G J M
Formato: Artigo
Lenguaje:Inglês
Publicado: BMJ Publishing Group 1996
Materias:
Papers
Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC408084/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16696100/
https://ncbi.nlm.nih.govhttps://doi.org/10.1136/mp.49.6.m340
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