Human papilloma virus detection by in situ hybridisation signal amplification based on biotinylated tyramine deposition

Aim—To describe a method for amplifying human papilloma virus (HPV) in situ hybridisation (ISH) signals. Methods—Three human cervical cell lines, namely CaSKi, HeLa and SiHa, containing different copy numbers of integrated HPV DNA were studied. Following ISH, catalysed reporter deposition (CARD), ba...

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Main Authors: Poddighe, P J, Bulten, J, Kerstens, H M J, Robben, J C M, Melchers, W J G, Hanselaar, A G J M
Format: Artigo
Jezik:Inglês
Izdano: 1996
Teme:
Papers
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC408084/
https://ncbi.nlm.nih.gov/pubmed/16696100
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