Human papilloma virus detection by in situ hybridisation signal amplification based on biotinylated tyramine deposition

Aim—To describe a method for amplifying human papilloma virus (HPV) in situ hybridisation (ISH) signals. Methods—Three human cervical cell lines, namely CaSKi, HeLa and SiHa, containing different copy numbers of integrated HPV DNA were studied. Following ISH, catalysed reporter deposition (CARD), ba...

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Autores principales: Poddighe, P J, Bulten, J, Kerstens, H M J, Robben, J C M, Melchers, W J G, Hanselaar, A G J M
Formato: Artigo
Lenguaje:Inglês
Publicado: 1996
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Papers
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC408084/
https://ncbi.nlm.nih.gov/pubmed/16696100
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