Nested PCR-SSCP assay for the detection of p53 mutations in paraffin wax embedded bone tumours: improvement of sensitivity and fidelity

DNA extraction and PCR amplification from paraffin wax embedded bone tumour specimens present several difficulties, firstly, because of the abundant matrix they contain and, secondly, because decalcification often causes degradation of DNA. In this report, comparative studies were carried out to det...

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Bibliografische gegevens
Hoofdauteurs: Wang, L T, Smith, A, Iacopetta, B, Wood, D J, Papadimitriou, J M, Zheng, M H
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1996
Onderwerpen:
Short Reports
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC408046/
https://ncbi.nlm.nih.gov/pubmed/16696068
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