Nested PCR-SSCP assay for the detection of p53 mutations in paraffin wax embedded bone tumours: improvement of sensitivity and fidelity

DNA extraction and PCR amplification from paraffin wax embedded bone tumour specimens present several difficulties, firstly, because of the abundant matrix they contain and, secondly, because decalcification often causes degradation of DNA. In this report, comparative studies were carried out to det...

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Detalhes bibliográficos
Main Authors: Wang, L T, Smith, A, Iacopetta, B, Wood, D J, Papadimitriou, J M, Zheng, M H
Formato: Artigo
Idioma:Inglês
Publicado em: 1996
Assuntos:
Short Reports
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC408046/
https://ncbi.nlm.nih.gov/pubmed/16696068
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