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Use of non-radioactive detection in SSCP, direct DNA sequencing and LOH analysis

Aims—To develop a protocol that is applicable to single strand conformation polymorphism (SSCP), direct sequencing and loss of heterozygosity analysis of DNA. Methods—The protocol is based on the detection of biotinylated DNA by a Streptavidin-alkaline phosphatase conjugate. Biotinylation of DNA was...

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Detalhes bibliográficos
Main Authors: Petersen, I, Reichel, M B, Dietel, M
Formato: Artigo
Idioma:Inglês
Publicado em: 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC408032/
https://ncbi.nlm.nih.gov/pubmed/16696052
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