A General NMR-Based Strategy for the in Situ Characterization of Sugar-Nucleotide-Dependent Biosynthetic Pathways

[Image: see text] A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively (13)C-labeled sugar nucleotides and inverse (13)C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents th...

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Bibliographic Details
Main Authors: Singh, Shanteri, Peltier-Pain, Pauline, Tonelli, Marco, Thorson, Jon S.
Format: Artigo
Language:Inglês
Published: American Chemical Society 2014
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4075999/
https://ncbi.nlm.nih.gov/pubmed/24911465
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ol501241a
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