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Cultivation to improve in vivo solubility of overexpressed arginine deiminases in Escherichia coli and the enzyme characteristics

BACKGROUND: Overexpression of foreign genes in Escherichia coli cells is an efficient means to obtain recombinant proteins. The technique is, however, often hampered by misfolding, degradation, aggregation and formation in inclusion bodies of products. RESULTS: In this study, we reported that in viv...

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Hlavní autoři: Wang, Ying, Li, Yue-Zhong
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4072490/
https://ncbi.nlm.nih.gov/pubmed/24908259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-53
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