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Cultivation to improve in vivo solubility of overexpressed arginine deiminases in Escherichia coli and the enzyme characteristics
BACKGROUND: Overexpression of foreign genes in Escherichia coli cells is an efficient means to obtain recombinant proteins. The technique is, however, often hampered by misfolding, degradation, aggregation and formation in inclusion bodies of products. RESULTS: In this study, we reported that in viv...
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
BioMed Central
2014
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4072490/ https://ncbi.nlm.nih.gov/pubmed/24908259 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-14-53 |
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