Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance

Pgp (P-glycoprotein) is a prototype ABC (ATP-binding-cassette) transporter involved in multidrug resistance of cancer. We used directed evolution to replace six cytoplasmic Cys (cysteine) residues in Pgp with all 20 standard amino acids and selected for active mutants. From a pool of 75000 transform...

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Detalhes bibliográficos
Main Authors: Swartz, Douglas J., Mok, Leo, Botta, Sri K., Singh, Anukriti, Altenberg, Guillermo A., Urbatsch, Ina L.
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2014
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Original Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4069687/
https://ncbi.nlm.nih.gov/pubmed/24825346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BSR20140062
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