Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance

Pgp (P-glycoprotein) is a prototype ABC (ATP-binding-cassette) transporter involved in multidrug resistance of cancer. We used directed evolution to replace six cytoplasmic Cys (cysteine) residues in Pgp with all 20 standard amino acids and selected for active mutants. From a pool of 75000 transform...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Swartz, Douglas J., Mok, Leo, Botta, Sri K., Singh, Anukriti, Altenberg, Guillermo A., Urbatsch, Ina L.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Portland Press Ltd. 2014
Gaiak:
Original Paper
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4069687/
https://ncbi.nlm.nih.gov/pubmed/24825346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BSR20140062
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